This suggests undesirable secondary interactions between the basic molecule and silanol groups on the stationary phase surface remain.įurther to the intermediate pH comparison (see Inertness-pH5.8 tab), the ACE SuperC18 again provides excellent peak shape and efficiency when analysing a basic molecule (pyridine) at high pH, indicating undesirable secondary interactions have been virtually eliminated. When analysing a basic molecule (pyridine) at high pH, differences in efficiency, peak tailing and retention are seen.

> The 'Porous ACE SuperC18 buffer guide' is available on the download tab.Ĭomparison of Column Inertness at High pH Traditional C18 bonded silica columns are prone to silica dissolution under such aggressive conditions, which can result in premature column deterioration. Under basic conditions with LC/MS compatible mobile phases (see Figure 2), the Encapsulated Bonding Technology (EBT™) shields the ACE SuperC18 surface from dissolution whilst maintaining excellent chromatographic performance. Many C18 bonded columns will exhibit ligand cleavage under acidic conditions, resulting in retention shifts and/or increased peak tailing. Under aggressive acidic conditions (see Figure 1) ACE SuperC18 is highly resistant to ligand cleavage, due to a combination of the Encapsulated Bonding Technology (EBT™) and ultra-inert ACE silica.

This higher ligand coverage results in improved inertness, chromatographic performance and stability. The unique Encapsulated Bonding Technology (EBT™) developed for ACE SuperC18 columns dramatically increases ligand coverage of the silica surface and effectively eliminates the effect of unbonded silanol groups from separations. Encapsulated Bonding Technology (EBT™) for improved chromatography and stability

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